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Twilight Stream is a biome added by Twilight Forest. Twilight Streams generate in-between main biomes in order to act as a natural barrier for different and unrelated biomes in the forest. Aside from that, it has no purpose and is effectively a river as seen in the overworld.
Twilight stream separating a Mushroom Forest and a Dark forest biome
Map view of Twilight Stream running through biomes
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| Twilight Forest Biomes | |
| Wet |  Fire Swamp • 16px Lake • 16px Swamp
|
|---|---|
| Forested | 16px Deep Mushroom Forest •  Dense Twilight Forest • 16px Dark Forest • 16px Enchanted Forest • 16px Mushroom Forest • 16px Twilight Forest • 16px Twilight Stream
|
| Non-Forested | 16px Clearing • 16px Highlands |
| Cold |  Snowy Forest • 16px Glacier
|
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